Part:BBa_K750010:Design

TIME DELAY 0.6:LuxI(RBS0.6)->LuxR->LuxPR->GFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  Illegal NheI site found at 1072
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 796
  Illegal BamHI site found at 65
  Illegal BamHI site found at 1012
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2027
  Illegal BsaI.rc site found at 2754
  

Design Notes

Lasr year, we use IPTG to induct the production of LuxI protein and LuxR protein. While this year we hope that there are an obvious process from dark to light. Because of the strong basal expression of promoter LuxPR(BBa_R0062), we have to change the promoter LuxPR(BBa_R0062) to Pbad(BBa_K206000).

Source

We built this part by biobricks from the DNA distribution kit plates 2012. The inspiration of design this part comes from further research of the iccdB project we designed last year.

References

[1]W.Claiborne Fuqua, S.C.W., E. Peter Greenberg, Quorum Sensing in Bacteria: the LuxR-LuxI Family of Cell Density-Responsive Transcriptional Regulatorst. JOURNAL OF BACTERIOLOGY, 1994. 176(2): p. 269-275.

[2]You, L., et al., Programmed population control by cell-cell communication and regulated killing. Nature, 2004. 428(6985): p. 868-71.

[3]Alon, U., Network motifs: theory and experimental approaches. Nat Rev Genet, 2007. 8(6): p. 450-61.

[4]Mangan, S., et al., The incoherent feed-forward loop accelerates the response-time of the gal system of Escherichia coli. J Mol Biol, 2006. 356(5): p. 1073-81.

[5]Camas, F.M., J. Blazquez, and J.F. Poyatos, Autogenous and nonautogenous control of response in a genetic network. Proc Natl Acad Sci U S A, 2006. 103(34): p. 12718-23.

[6]http://2011.igem.org/Team:XMU-China